Applied Scientific Instruments Co., Ltd.

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Nanoparticle Size And Zeta Potential Analyzers

หมวดหมู่สินค้า: Bettersize
รหัส : BeNano 180 Zeta Pro
The BeNano Series is the latest generation of nanoparticle size and zeta potential analyzers designed by Bettersize Instruments Ltd. Dynamic light scattering (DLS), electrophoretic light scattering (ELS) and static light scattering (SLS) are integrated into the system to provide accurate measurements on particle size, zeta potential and molecular weight.

09 มีนาคม 2566

ผู้ชม 411 ผู้ชม

Summary

1) Optical Layout of the BeNano Series

Optical System of the BeNano 180 Zeta Pro

2) Dynamic Light Scattering
Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS) or quasi-elastic light scattering (QELS), is a technology used to detect the fluctuations of the scattering intensities caused by the Brownian motion of particles.

In the dispersant, smaller particles move faster, while larger particles move slower. An avalanche photodiode (APD) detector aligned at 90°collects the scattering intensities of the particles and records them with time. The time-dependent fluctuation is converted into a correlation function using the correlator. By applying a mathematic algorithm, the diffusion coefficient D is thereby obtained. The hydrodynamic diameter D H and its distribution are calculated through the Stokes-Einstein equation.

3) Electrophoretic Light Scattering
Particles usually carry charges on the surface in aqueous systems, surrounded by counter-ions that form a firmly inner Stern layer and an outer shear layer. Zeta potential is the electrical potential at the interface of the shear layer. Suspension systems with higher zeta potentials tend to be more stable and less likely to form aggregates.
Electrophoretic light scattering (ELS) is a technology for measuring electrophoretic mobility via Doppler shifts of the scattered light. When an incident light illuminates dispersed particles that are subjected to an applied electric field, the frequency of the particles' scattered light will be different from the incident light due to the Doppler effect. The frequency shift is measured and converted to provide the electrophoretic mobility and hence the zeta potential of a sample by Henry's equation.

4) Static Light Scattering
Static light scattering (SLS) is a technology which measures the scattering intensities of the sample, weight-average molecular weight (Mw) and second virial coefficient A2 through Rayleigh equation:

where C is the sample concentration, θ is the detection angle, Rθ is the Rayleigh ratio used to characterize the intensity ratio between the scattered light and the incident light at angle θ, Mw is the sample’s weight-average molecular weight, A2 is the second virial coefficient, and K is a constant related to (dn/dc)2.
During molecular weight measurements, scattering intensities of the sample at different concentrations are detected. By using the scattering intensity and Rayleigh ratio of a known standard (such as toluene), the Rayleigh ratios of samples at different concentrations are computed and plotted into a Debye plot. The molecular weight and the second virial coefficient are then obtained through the intercept and slope from the linear regression of the Debye plot.

5)Backscattering Detection Technology
Features
Higher detection sensitivity for samples with very low concentrations down to 0.1 ppm.
Intelligent search for the optimal detection position, which greatly avoids the multiple scattering effect of samples and can detect samples with concentrations of up to 40%.
Effectively limiting the interference of dust.

When the sample is detected at 173° at the middle of the cell, the scattered volume is 8-10 times larger than at 90°, leading to much higher sensitivity, and avoiding reflections from the sample cell wall.

When measuring concentrated size standards, the results obtained from the 173° detector are in much better agreement with the nominal values, compared with the results from the 90° detector.

6)Phase Analysis Light Scattering (PALS)
The traditional ELS converts the correlated scattering signals into frequency distribution and then calculates the frequency shift Δf of the scattered light, compared with the reference light. Phase analysis light scattering (PALS), an advanced technology based on the traditional ELS technology, has been further developed by Bettersize Instruments Ltd. to measure zeta potential and its distribution of a sample. By analyzing the phase information Φ of the original scattered signal, PALS obtains the frequency information of that light. The phase shift with time dΦ/dt is proportional to the frequency shift Δf. PALS technology can suppress the influence of the Brownian motion of particles on the results, thereby providing higher statistical accuracy. In various applications, PALS can effectively measure the zeta potential of particles whose charge approaches the isoelectric point, for instance, particles with very slow electrophoretic mobility at a high salt concentration.

7) Temperature Trend Measurement
Temperature trend measurement includes:
Size vs. Temperature
Zeta Potential vs. Temperature

Investigating the particle size and zeta potential of the samples under different temperatures is significant in many applications. The function of Programmed Temperature Change, ranging from -10°C to 110°C, makes Temperature Trend Measurement available in the BeNano Series.

Benefits
This feature benefits users who need to study the stability of protein formulations. Generally, the higher the denaturation temperature of the protein, the more stable the formulation.
Besides, it is useful for users who need to simulate real-time aging using elevated temperatures to manually speed up the aging process.

8) Viscosity Measurement
For the sample of unknown viscosity, the viscosity measurement could be implemented using tracer particles with known sizes (e.g., standard samples with nominal sizes). When the measurement ends, input the accurate size of the tracer particles, and the viscosity of the sample could be determined.

After the measurement, choose and right-click on the corresponding result. Click "Viscosity Calculator" on the pop-up menu.
Through inputting the nominal values of the tracer particles and clicking on "Calculation", the viscosity of the sample could be finally ascertained.

Application

Dynamic Light Scattering (DLS)-Applications

- Particle size and distribution of polymers, colloids, self-assembling system, biomacromolecules, proteins, peptides, antigens, antibodies, nano metal/non-metal particles.
- Studies on the polymerization process and reaction mechanisms.
- Studies on kinetics of self-assembly and other processes of polymerization and depolymerization of macromolecules.
- Research on thermal-sensitive systems, for example, PNIPAm polymer.

Electrophoretic Light Scattering(ELS)-Applications

- Suspension systems such as macromolecules, colloids, emulsions, coal-water slurries, proteins, antigens, antibodies and nanometal/non-metal particles.
- Industries include, but are not limited to, chemicals, chemical engineering, biology, food and beverage, pharmaceuticals, water treatment, environmental protection, abrasive, and paints.
- Monitoring and controlling product stability.
- Stability research and control of the suspension system.
- Studies on the surface electrical properties and surface modifications.

Static Light Scattering(SLS)-Applications

- Chemical engineering: characterization of polymers, micelles and supermolecules.
- Petroleum engineering: characterization of macromolecule additives and oil-displacing surfactants
- Life science: characterization of proteins, polypeptides, and polysaccharides.
- Pharmaceuticals: research on aggregation and stability of drugs.
- Conformation of supermolecules, research on self-assembling aggregates.

Specification

Functions

Parameter

BeNano 180 Zeta Pro

BeNano 180 Zeta

BeNano 90 Zeta

BeNano Zeta

BeNano 180 Pro

BeNano 180

BeNano 90

Size
measurement

Size
measurement range

0.3 nm - 15 μm*

0.3 nm - 10 μm*

0.3 nm - 15 μm*

N/A

0.3 nm - 15 μm*

0.3 nm -10 μm*

0.3 nm - 15 μm*

Sample volume

3 μL - 1 mL*

40 μL - 1 mL*

3 μL - 1 mL*

N/A

3 μL - 1 mL*

40 μL - 1 mL*

3 μL - 1 mL*

Detection angle

90° & 173° & 12°

173° & 12°

90° & 12°

N/A

90° & 173°

173°

90°

Analysis algorithm

Cumulants, Universal Mode,
CONTIN

Cumulants, Universal Mode,
CONTIN

Cumulants, Universal Mode,
CONTIN

N/A

Cumulants, Universal Mode,
CONTIN

Cumulants, Universal Mode,
CONTIN

Cumulants, Universal Mode,
CONTIN

Upper limit of
concentration range

40% w/v*

40% w/v*

Optical clear+

N/A

40% w/v*

40% w/v*

Optical clear†

Detection position

Movable position
0 - 5 mm

Movable position
0 - 5 mm

Fixed position
5 mm

N/A

Movable position
0 - 5 mm

Movable position
0 - 5 mm

Fixed position
5 mm

Zeta potential
measurement

Detection angle

12°

12°

12°

12°

N/A

N/A

N/A

Zeta potential
measurement range

No actual limitation

No actual limitation

No actual limitation

No actual limitation

N/A

N/A

N/A

Electrophoretic mobility

> ± 20 μm·cm/V·s

> ± 20 μm·cm/V·s

> ± 20 μm·cm/V·s

> ± 20 μm·cm/V·s

N/A

N/A

N/A

Conductivity

0 - 260 mS/cm

0 - 260 mS/cm

0 - 260 mS/cm

0 - 260 mS/cm

N/A

N/A

N/A

Sample volume

0.75 - 1 mL

0.75 - 1 mL

0.75 - 1 mL

0.75 - 1 mL

N/A

N/A

N/A

Sample size

2 nm - 110 μm

2 nm - 110 μm

2 nm - 110 μm

2 nm - 110 μm

N/A

N/A

N/A

Other
measurements

Molecular weight
(Mw)

342 Da - 2 x 107Da*

342 Da - 2 x 107Da*

342 Da - 2 x 107Da*

N/A

342 Da - 2 x 107Da*

342 Da - 2 x 107Da*

342 Da - 2 x 107Da*

Viscosity

0.01 cp - 100 cp*

0.01 cp - 100 cp*

0.01 cp - 100 cp*

N/A

0.01 cp - 100 cp*

0.01 cp - 100 cp*

0.01 cp - 100 cp*

Interaction parameter
KD

No actual limitation

No actual limitation

No actual limitation

N/A

No actual limitation

No actual limitation

No actual limitation

Trend measurement

Time and temperature

Time and temperature

Time and temperature

Time and temperature

Time and temperature

Time and temperature

Time and temperature

System
parameters

Temperature
control range

-10℃ - 110℃,
±0.1℃

-10℃ - 110℃,
±0.1℃

-10℃ - 110℃,
±0.1℃

-10℃ - 110℃,
±0.1℃

-10℃ - 110℃,
±0.1℃

-10℃ - 110℃,
±0.1℃

-10℃ - 110℃,
±0.1℃

Condensation control

Dry air or nitrogen

Dry air or nitrogen

Dry air or nitrogen

Dry air or nitrogen

Dry air or nitrogen

Dry air or nitrogen

Dry air or nitrogen

Laser source

50 mW Solid-state laser, 671 nm

50 mW Solid-state laser, 671 nm

50 mW Solid-state laser, 671 nm

50 mW Solid-state laser, 671 nm

50 mW Solid-state laser, 671 nm

50 mW Solid-state laser, 671 nm

50 mW Solid-state laser, 671nm

Correlator

Up to 4000 channels,

1011linear
dynamic range

Up to 4000 channels,

1011linear
dynamic range

Up to 4000 channels,

1011linear
dynamic range

Up to 4000 channels,

1011linear
dynamic range

Up to 4000 channels,

1011linear
dynamic range

Up to 4000 channels,

1011linear
dynamic range

Up to 4000 channels,

1011linear
dynamic range

Detector

Avalanche photodiode
(APD)

Avalanche photodiode
(APD)

Avalanche photodiode
(APD)

Avalanche photodiode
(APD)

Avalanche photodiode
(APD)

Avalanche photodiode
(APD)

Avalanche photodiode
(APD)

Intensity control

0.0001% - 100%,
manual or automatic

0.0001% - 100%,
manual or automatic

0.0001% - 100%,
manual or automatic

0.0001% - 100%,
manual or automatic

0.0001% - 100%,
manual or automatic

0.0001% - 100%,
manual or automatic

0.0001% - 100%,
manual or automatic

Dimensions
(L x W x H)

62.5 x 40 x 24.5 cm
(22 kg)

62.5 x 40 x 24.5 cm
(22 kg)

62.5 x 40 x 24.5 cm
(22 kg)

62.5 x 40 x 24.5 cm
(22 kg)

62.5 x 40 x 24.5 cm
(22 kg)

62.5 x 40 x 24.5 cm
(22 kg)

62.5 x 40 x 24.5 cm
(22 kg)

Power supply

AC 100-240 V,
50-60 Hz, 4A

AC 100-240 V,
50-60 Hz, 4A

AC 100-240 V,
50-60 Hz, 4A

AC 100-240 V,
50-60 Hz, 4A

AC 100-240 V,
50-60 Hz, 4A

AC 100-240 V,
50-60 Hz, 4A

AC 100-240 V,
50-60 Hz, 4A

Conformity
to standards

ISO 13321, ISO 22412-2017, ISO 13099-1, ISO 13099-2

ISO 13321, ISO 22412-2017, ISO 13099-1, ISO 13099-2

ISO 13321, ISO 22412-2017, ISO 13099-1, ISO 13099-2

ISO 13321, ISO 22412-2017, ISO 13099-1, ISO 13099-2

ISO 13321, ISO 22412-2017, ISO 13099-1, ISO 13099-2

ISO 13321, ISO 22412-2017, ISO 13099-1, ISO 13099-2

ISO 13321, ISO 22412-2017, ISO 13099-1, ISO 13099-2

Accessories

Disposable
micro-volume cuvette

40 - 50 μL

40 - 50 μL

40 - 50 μL

N/A

40 - 50 μL

40 - 50 μL

40 - 50 μL

Micro-volume
glass cuvette

25 μL

N/A

25 μL

N/A

25 μL

N/A

25 μL

Glass cuvette
with round opening

1 mL

1 mL

1 mL

N/A

1 mL

1 mL

1 mL

Capillary sizing cell

3 - 5 μL

N/A

3 - 5 μL

N/A

3 - 5 μL

N/A

3 - 5 μL

Dip cell kit

1 - 1.5 mL,
zeta potential measurement
for organic-based samples

1 - 1.5 mL,
zeta potential measurement
for organic-based samples

1 - 1.5 mL,
zeta potential measurement
for organic-based samples

1 - 1.5 mL,
zeta potential measurement
for organic-based samples

N/A

N/A

N/A

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